CD63 Mouse Monoclonal Antibody [Clone ID: OTI2G6]

CAT#: CF802751

Carrier-free (BSA/glycerol-free) CD63 mouse monoclonal antibody, clone OTI2G6 (formerly 2G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (RC201733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63. Positive lysates LY419757 (100ug) and LC419757 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from MCF7 cell line by using anti-CD63 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802751)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802751)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802751)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802751)

• HEK293T cells transfected with either RC201733 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD63 antibody (TA802751), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living A549 cells, using anti-CD63 antibody (TA802751), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of MCF-7 cells, using anti-CD63 antibody (TA802751), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100)

Show all 9 images

Specifications

Product Data

• Clone Name: OTI2G6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD63 (NP_001771) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.5 kDa
• Gene Name: Homo sapiens CD63 molecule (CD63), transcript variant 1, mRNA.
• Database Link:
  NP_001771
  Entrez Gene 967 Human
  UniProt ID P08962
• Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
• Synonyms: LAMP-3; ME491; MLA1; OMA81H; TSPAN30

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome