TIF1 alpha (TRIM24) Mouse Monoclonal Antibody [Clone ID: OTI3A8]

CAT#: CF802789

Carrier-free (BSA/glycerol-free) TRIM24 mouse monoclonal antibody, clone OTI3A8 (formerly 3A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24.

Specifications

Product Data

• Clone Name: OTI3A8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 706-1016 of human TRIM24 (NP_003843) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens tripartite motif containing 24 (TRIM24), transcript variant 2, mRNA.
• Database Link:
  NP_003843
  Entrez Gene 21848 MouseEntrez Gene 8805 Human
  UniProt ID O15164
• Background: The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors