EZH2 Mouse Monoclonal Antibody [Clone ID: OTI2B10]

CAT#: CF802907

Carrier-free (BSA/glycerol-free) EZH2 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EZH2 (RC202054, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EZH2. Positive lysates LY401416 (100ug) and LC401416 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and EZH2-Knockout Hela cells (KO, Cat# LC810075) were separated by SDS-PAGE and immunoblotted with anti-EZH2 monoclonal antibody TA802907, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EZH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802907)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-EZH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802907)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-EZH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802907)

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Specifications

Product Data

• Clone Name: OTI2B10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 182-431 of human EZH2 ( NP_004447) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.8 kDa
• Gene Name: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 1, mRNA.
• Database Link:
  NP_004447
  Entrez Gene 14056 MouseEntrez Gene 312299 RatEntrez Gene 2146 Human
  UniProt ID Q15910
• Background: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
• Synonyms: ENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors