AVPI1 Mouse Monoclonal Antibody [Clone ID: OTI1G1]

CAT#: CF802924

Carrier-free (BSA/glycerol-free) AVPI1 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AVPI1 (RC201721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AVPI1. Positive lysates LY402873 (100ug) and LC402873 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AVPI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802924)

Specifications

Product Data

• Clone Name: OTI1G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AVPI1 (NP_068378) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.6 kDa
• Gene Name: Homo sapiens arginine vasopressin induced 1 (AVPI1), mRNA.
• Database Link:
  NP_068378
  Entrez Gene 60370 Human
  UniProt ID Q5T686
• Synonyms: PP5395; VIP32; VIT32