CD68 Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF802949

Carrier-free (BSA/glycerol-free) CD68 mouse monoclonal antibody, clone OTI3B6 (formerly 3B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD68 (RC200392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD68. Positive lysates LY420047 (100ug) and LC420047 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD68 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802949)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD68 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802949)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CD68 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802949)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD68 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802949)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CD68 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802949)

• Flow cytometric Analysis of Uacc257 cells, using anti-CD68 antibody (TA802949), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue). Dilution: 1:50

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Specifications

Product Data

• Clone Name: OTI3B6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-319 of human CD68 (NP_001242)produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.2 kDa
• Gene Name: Homo sapiens CD68 molecule (CD68), transcript variant 1, mRNA.
• Database Link:
  NP_001242
  Entrez Gene 968 Human
  UniProt ID P34810
• Background: This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: GP110; LAMP4; SCARD1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome