p16INK4A (CDKN2A) Mouse Monoclonal Antibody [Clone ID: OTI1D3]

CAT#: CF802963

Carrier-free (BSA/glycerol-free) CDKN2A mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A (RC220937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2A. Positive lysates LY400022 (100ug) and LC400022 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CDKN2A monoclonal antibody at 1:200.

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  Immunocytochemistry staining of MDA-MB-468 cells using anti-CDKN2A mouse monoclonal antibody (TA802963). The right is MCF7 cells as negative control (1:50).

Specifications

Product Data

• Clone Name: OTI1D3
• Applications: IF, WB
• Recommended Dilution: WB 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDKN2A (NP_000068) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.4 kDa
• Gene Name: cyclin dependent kinase inhibitor 2A
• Database Link:
  NP_000068
  Entrez Gene 1029 Human
  UniProt ID Q8N726
• Synonyms: ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer