TIPIN Mouse Monoclonal Antibody [Clone ID: OTI2C10]

CAT#: CF802975

Carrier-free (BSA/glycerol-free) TIPIN mouse monoclonal antibody, clone OTI2C10 (formerly 2C10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPIN (RC201502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPIN. Positive lysates LY402647 (100ug) and LC402647 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TIPIN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802975)

Specifications

Product Data

• Clone Name: OTI2C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TIPIN (NP_060328) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.3 kDa
• Gene Name: Homo sapiens TIMELESS interacting protein (TIPIN), transcript variant 1, mRNA.
• Database Link:
  NP_060328
  Entrez Gene 54962 Human
  UniProt ID Q9BVW5
• Synonyms: FLJ20516

Reference Data

• Protein Families: Druggable Genome