CD79A Mouse Monoclonal Antibody [Clone ID: OTI3D3]

CAT#: CF803156

Carrier-free (BSA/glycerol-free) CD79A mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD79A (RC220359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD79A. Positive lysates LY400674 (100ug) and LC400674 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 different cell lines and human spleen tissue lysate by using anti-CD79A monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD79A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803156)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD79A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803156)

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  Flow cytometric Analysis of Jurkat cells, using anti-CD79A antibody (TA803156), (Red), compared to anti-CD3 antibody (TA807198), (Blue) (1:100).

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  Flow cytometric Analysis of Ramos cells, using anti-CD79A antibody (TA803156), (Red), compared to anti-CD3 antibody (TA807198), (Blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI3D3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD79A (NP_001774) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens CD79a molecule (CD79A), transcript variant 1, mRNA.
• Database Link:
  NP_001774
  Entrez Gene 973 Human
  UniProt ID P11912
• Background: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
• Synonyms: IGA; MB-1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Primary immunodeficiency