CEACAM5 Mouse Monoclonal Antibody [Clone ID: OTI2B12]

CAT#: CF803181

Carrier-free (BSA/glycerol-free) CEACAM5 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CEACAM5 (RC206434, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CEACAM5.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA803181)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA803181)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA803181)

Specifications

Product Data

• Clone Name: OTI2B12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 35-680 of human CEACAM5 (NP_004354) produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens carcinoembryonic antigen related cell adhesion molecule 5 (CEACAM5), transcript variant 1, mRNA.
• Database Link:
  NP_004354
  Entrez Gene 1048 Human
  UniProt ID P06731
• Background: Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770). [supplied by OMIM, Oct 2009]
• Synonyms: CD66e; CEA