ROR alpha (RORA) Mouse Monoclonal Antibody [Clone ID: OTI2E8]

CAT#: CF803200

Carrier-free (glycerol/BSA-free) RORA mouse monoclonal antibody, clone OTI2E8 (formerly 2E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RORA (RC214618, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RORA. Positive lysates LY401041 (100ug) and LC401041 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 98-345 of human RORA (NP_002934) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.1 kDa
• Gene Name: Homo sapiens RAR related orphan receptor A (RORA), transcript variant 3, mRNA.
• Database Link:
  NP_002934
  Entrez Gene 19883 MouseEntrez Gene 300807 RatEntrez Gene 6095 Human
  UniProt ID P35398
• Background: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: NR1F1; ROR1; ROR2; ROR3; RZR-ALPHA; RZRA

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors