ROR alpha (RORA) Mouse Monoclonal Antibody [Clone ID: OTI5A6]

CAT#: CF803241

Carrier-free (BSA/glycerol-free) RORA mouse monoclonal antibody,clone OTI5A6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RORA (RC214618, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RORA (1:2000). Positive lysates LY401041 (100ug) and LC401041 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5A6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 98-345 of human RORA (NP_002934) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.1 kDa
• Gene Name: Homo sapiens RAR related orphan receptor A (RORA), transcript variant 3, mRNA.
• Database Link:
  NP_002934
  Entrez Gene 19883 MouseEntrez Gene 300807 RatEntrez Gene 6095 Human
  UniProt ID P35398
• Synonyms: NR1F1; ROR1; ROR2; ROR3; RZR-ALPHA; RZRA

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors