Von Willebrand Factor (VWF) Mouse Monoclonal Antibody [Clone ID: OTI8A6]

CAT#: CF803275

Carrier-free (BSA/glycerol-free) VWF mouse monoclonal antibody, clone OTI8A6 (formerly 8A6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWF (RC218497, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWF (1:500).

Specifications

Product Data

• Clone Name: OTI8A6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 764-1263 of human VWF (NP_000543) produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 309.3 kDa
• Gene Name: Homo sapiens von Willebrand factor (VWF), mRNA.
• Database Link:
  NP_000543
  Entrez Gene 7450 Human
  UniProt ID P04275
• Background: The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
• Synonyms: F8VWF; VWD

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, ECM-receptor interaction, Focal adhesion