NR2C1 Mouse Monoclonal Antibody [Clone ID: OTI2E2]

CAT#: CF803355

Carrier-free (BSA/glycerol-free) NR2C1 mouse monoclonal antibody, clone OTI2E2 (formerly 2E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C1 (RC208060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2C1. Positive lysates LY422297 (100ug) and LC422297 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-237 of human NR2C1 (NP_001027458) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: Homo sapiens nuclear receptor subfamily 2 group C member 1 (NR2C1), transcript variant 2, mRNA.
• Database Link:
  NP_001027458
  Entrez Gene 22025 MouseEntrez Gene 252924 RatEntrez Gene 7181 Human
  UniProt ID P13056
• Background: This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: TR2

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors