VEGFA Mouse Monoclonal Antibody [Clone ID: OTI2E11]

CAT#: CF803421

Carrier-free (BSA/glycerol-free) VEGFA mouse monoclonal antibody,clone OTI2E11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFA (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFA (1:500).

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  HEK293T cells were transfected with the overexpression plasmids of 9 VEGF isoforms (from left to right:VEGF206, RC223789; VEGF189, RC229706; L-VEGF165, RC223884; VEGF165, RC229662; L-VEGF121, RC222129; VEGF iso18, RC229874; VEGF183, RC229686; VEGF145, RC231952; L-VEGF189, RC224244) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (TA180144, 1:1000) or anti-VEGFA mouse monoclonal antibody. (TA803421, 1:500)

Specifications

Product Data

• Clone Name: OTI2E11
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 206-412 of human VEGFA (NP_001020537) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.
• Database Link:
  NP_001020537
  Entrez Gene 7422 Human
  UniProt ID P15692
• Synonyms: MVCD1; VEGF; VPF

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway