MSRB3 Mouse Monoclonal Antibody [Clone ID: OTI6F9]

CAT#: CF803471

Carrier-free (BSA/glycerol-free) MSRB3 mouse monoclonal antibody, clone OTI6F9 (formerly 6F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSRB3 (RC216512, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSRB3. Positive lysates LY403694 (100ug) and LC403694 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6F9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 33-192 of human MSRB3 (NP_932346) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.5 kDa
• Gene Name: Homo sapiens methionine sulfoxide reductase B3 (MSRB3), transcript variant 1, mRNA.
• Database Link:
  NP_932346
  Entrez Gene 253827 Human
  UniProt ID Q8IXL7
• Background: The protein encoded by this gene catalyzes the reduction of methionine sulfoxide to methionine. This enzyme acts as a monomer and requires zinc as a cofactor. Several transcript variants encoding two different isoforms have been found for this gene. One of the isoforms localizes to mitochondria while the other localizes to endoplasmic reticula. [provided by RefSeq, Jul 2010]
• Synonyms: DFNB74