MSMB Mouse Monoclonal Antibody [Clone ID: OTI4C1]

CAT#: CF803526

Carrier-free (BSA/glycerol-free) MSMB mouse monoclonal antibody, clone OTI4C1 (formerly 4C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSMB (RC202704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSMB. Positive lysates LY400872 (100ug) and LC400872 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MSMB mouse monoclonal antibody. (TA803526) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MSMB mouse monoclonal antibody. (TA803526) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI4C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified prostate specific antigen (MSMB) from human seminal fluid
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10.7 kDa
• Gene Name: Homo sapiens microseminoprotein beta (MSMB), transcript variant PSP94, mRNA.
• Database Link:
  NP_002434
  Entrez Gene 4477 Human
  UniProt ID P08118
• Background: The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HPC13; IGBF; MSP; MSPB; PN44; PRPS; PSP; PSP-94; PSP57; PSP94

Reference Data

• Protein Families: Secreted Protein, Transmembrane