CD63 Mouse Monoclonal Antibody [Clone ID: OTI5E5]

CAT#: CF803574

Carrier-free (BSA/glycerol-free) CD63 mouse monoclonal antibody, clone OTI5E5 (formerly 5E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (RC201733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63. Positive lysates LY419757 (100ug) and LC419757 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803574)

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Specifications

Product Data

• Clone Name: OTI5E5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD63 (NP_001771) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.5 kDa
• Gene Name: Homo sapiens CD63 molecule (CD63), transcript variant 1, mRNA.
• Database Link:
  NP_001771
  Entrez Gene 967 Human
  UniProt ID P08962
• Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
• Synonyms: LAMP-3; ME491; MLA1; OMA81H; TSPAN30

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome