CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: OTI6H9]

CAT#: CF803640

Carrier-free (BSA/glycerol-free) TFRC mouse monoclonal antibody,clone OTI6H9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFRC (RC200980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFRC (1:500). Positive lysates LY418818 (100ug) and LC418818 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 2 cell lines and 1 tissue lysate by using anti-TFRC monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI6H9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TFRC (NP_003225) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.7 kDa
• Gene Name: Homo sapiens transferrin receptor (TFRC), transcript variant 1, mRNA.
• Database Link:
  NP_003225
  Entrez Gene 7037 Human
  UniProt ID P02786
• Synonyms: CD71; IMD46; p90; T9; TFR; TFR1; TR; TRFR

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Endocytosis, Hematopoietic cell lineage