Acid sphingomyelinase (SMPD1) Mouse Monoclonal Antibody [Clone ID: OTI3H7]

CAT#: CF803659

Carrier-free (BSA/glycerol-free) SMPD1 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1 (RC219758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMPD1. Positive lysates LY400191 (100ug) and LC400191 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803659)

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Specifications

Product Data

• Clone Name: OTI3H7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 62-341 of human SMPD1 (NP_000534) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA.
• Database Link:
  NP_000534
  Entrez Gene 6609 Human
  UniProt ID P17405
• Background: The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
• Synonyms: ASM; ASMASE; NPD

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism