LYRIC (MTDH) Mouse Monoclonal Antibody [Clone ID: OTI2H4]

CAT#: CF803709

Carrier-free (BSA/glycerol-free) MTDH mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTDH (RC207238, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTDH. Positive lysates LY403614 (100ug) and LC403614 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-MTDH monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MTDHmouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803709)

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Specifications

Product Data

• Clone Name: OTI2H4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 70-582 of human MTDH (NP_848927)produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens metadherin (MTDH), transcript variant 1, mRNA.
• Database Link:
  NP_848927
  Entrez Gene 67154 MouseEntrez Gene 170910 RatEntrez Gene 92140 Human
  UniProt ID Q86UE4
• Synonyms: 3D3; AEG-1; AEG1; LYRIC

Reference Data

• Protein Families: Transmembrane