Fibronectin (FN1) Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CAT#: CF803782

Carrier-free (BSA/glycerol-free) FN1 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FN1 (RC212860, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FN1. Positive lysates LY409278 (100ug) and LC409278 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-FN1 mouse monoclonal antibody. (TA803782) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI3F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-307 of human FN1 (NP_473375) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens fibronectin 1 (FN1), transcript variant 7, mRNA.
• Database Link:
  NP_473375
  Entrez Gene 14268 MouseEntrez Gene 25661 RatEntrez Gene 2335 Human
  UniProt ID P02751
• Background: This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer