RTRAF Mouse Monoclonal Antibody [Clone ID: OTI2A4]

CAT#: CF803840

Carrier-free (BSA/glycerol-free) C14orf166 mouse monoclonal antibody, clone OTI2A4 (formerly 2A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C14orf166 (RC200016, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C14orf166. Positive lysates LY414235 (100ug) and LC414235 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-C14orf166 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-C14orf166 mouse monoclonal antibody. (TA803840) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI2A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C14orf166 (NP_057123) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens RNA transcription, translation and transport factor (RTRAF), mRNA.
• Database Link:
  NP_057123
  Entrez Gene 51637 Human
  UniProt ID Q9Y224
• Synonyms: CGI-99; CGI99; CLE; CLE7; hCLE1; LCRP369; RLLM1