ACE2 Mouse Monoclonal Antibody [Clone ID: OTI2G7] (Angiotensin Converting Enzyme 2)

CAT#: CF803845

Carrier-free (BSA/glycerol-free) ACE2 mouse monoclonal antibody,clone OTI2G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE2 (RC208442, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACE2 (1:2000).

• 

  Western blot analysis of extracts (35ug) from human kidney tissue lysate by using anti-ACE2 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA803845) (1:500)

Specifications

Product Data

• Clone Name: OTI2G7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 18-237 of human ACE2 (NP_068576) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens angiotensin I converting enzyme 2 (ACE2), mRNA.
• Database Link:
  NP_068576
  Entrez Gene 59272 Human
  UniProt ID Q9BYF1
• Synonyms: ACEH

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system