ARIH2 Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CAT#: CF804094

Carrier-free (BSA/glycerol-free) ARIH2 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARIH2 (RC201846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARIH2. Positive lysates LY416714 (100ug) and LC416714 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3F9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 201-493 of human ARIH2 (NP_006312) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.6 kDa
• Gene Name: Homo sapiens ariadne RBR E3 ubiquitin protein ligase 2 (ARIH2), transcript variant 2, mRNA.
• Database Link:
  NP_006312
  Entrez Gene 23807 MouseEntrez Gene 316005 RatEntrez Gene 10425 Human
  UniProt ID O95376
• Synonyms: ARI2; TRIAD1

Reference Data

• Protein Families: Druggable Genome