TACC2 Mouse Monoclonal Antibody [Clone ID: OTI4F10]

CAT#: CF804145

Carrier-free (BSA/glycerol-free) TACC2 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)

Product Images

Western blot analysis of 293T (left) and MCF7 (right) cell lysate (35ug) by using anti-TACC2 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-TACC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804145)

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-TACC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804145)

Specifications

Product Data

• Clone Name: OTI4F10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 727-1026 of human TACC2 (NP_008928) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens transforming acidic coiled-coil containing protein 2 (TACC2), transcript variant 4, mRNA.
• Database Link:
  NP_008928
  Entrez Gene 10579 Human
  UniProt ID O95359
• Synonyms: AZU-1; ECTACC