FAF1 Mouse Monoclonal Antibody [Clone ID: OTI11H10]

CAT#: CF804157

Carrier-free (BSA/glycerol-free) FAF1 mouse monoclonal antibody, clone OTI11H10 (formerly 11H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Positive lysates LY416234 (100ug) and LC416234 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of HT29 cell lysate (35ug) by using anti-FAF1 monoclonal antibody. Dilution: 1:500

Specifications

Product Data

• Clone Name: OTI11H10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 415-650 of human FAF1 (NP_008982) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.8 kDa
• Gene Name: Homo sapiens Fas associated factor 1 (FAF1), mRNA.
• Database Link:
  NP_008982
  Entrez Gene 14084 MouseEntrez Gene 140657 RatEntrez Gene 11124 Human
  UniProt ID Q9UNN5
• Background: Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by this gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by this gene requires a ubiquitin-like domain but not the FAS-binding domain. [provided by RefSeq, Jul 2008]
• Synonyms: CGI-03; hFAF1; HFAF1s; UBXD12; UBXN3A

Reference Data

• Protein Families: Druggable Genome