Macrophage Inflammatory Protein 3 beta (CCL19) Mouse Monoclonal Antibody [Clone ID: OTI2A12]

CAT#: CF804261

Carrier-free (glycerol/BSA-free) CCL19 mouse monoclonal antibody, clone OTI2A12 (formerly 2A12)

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CCL19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804261)

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Specifications

Product Data

• Clone Name: OTI2A12
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-98 of human CCL19 (NP_006265) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 8.8 kDa
• Gene Name: Homo sapiens C-C motif chemokine ligand 19 (CCL19), mRNA.
• Database Link:
  NP_006265
  Entrez Gene 6363 Human
  UniProt ID Q99731
• Background: This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Jul 2008]
• Synonyms: CKb11; ELC; MIP-3b; MIP3B; SCYA19

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction