Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: OTI8E9]

CAT#: CF804315

Carrier-free (BSA/glycerol-free) ESR1 mouse monoclonal antibody, clone OTI8E9 (formerly 8E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ESR1 (RC213277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESR1. Positive lysates LY400046 (100ug) and LC400046 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ESR1 mouse monoclonal antibody. (TA804315)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804315) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804315) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804315) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804315) (1:150)

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Specifications

Product Data

• Clone Name: OTI8E9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-310 of human ESR1 (NP_000116) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66 kDa
• Gene Name: Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1, mRNA.
• Database Link:
  NP_000116
  Entrez Gene 13982 MouseEntrez Gene 24890 RatEntrez Gene 2099 Human
  UniProt ID P03372
• Synonyms: ER; Era; ESR; ESRA; ESTRR; NR3A1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors