PWWP3A Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF804374

Carrier-free (BSA/glycerol-free) MUM1 mouse monoclonal antibody,clone OTI1B2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MUM1 (RC211098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MUM1. Positive lysates LY409887 (100ug) and LC409887 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-270 of human MUM1 (NP_116242) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.6 kDa
• Gene Name: Homo sapiens PWWP domain containing 3A, DNA repair factor (PWWP3A), transcript variant 1, mRNA.
• Database Link:
  NP_116242
  Entrez Gene 84939 Human
  UniProt ID Q2TAK8
• Synonyms: EXPAND1; HSPC211; MUM-1