CD34 Mouse Monoclonal Antibody [Clone ID: OTI39B1]

CAT#: CF804414

Carrier-free (glycerol/BSA-free) CD34 mouse monoclonal antibody, clone OTI39B1 (formerly 39B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD34 (RC204446, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD34. Positive lysates LY422593 (100ug) and LC422593 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804414)

Specifications

Product Data

• Clone Name: OTI39B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:200, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: HEK293T cells transfected with full length human CD34 gene (RC204446)
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.5 kDa
• Gene Name: Homo sapiens CD34 molecule (CD34), transcript variant 1, mRNA.
• Database Link:
  NP_001020280
  Entrez Gene 947 Human
  UniProt ID P28906
• Background: The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
• Synonyms: CD34 antigen; CD34 molecule; hematopoietic progenitor cell antigen CD34; OTTHUMP00000034733; OTTHUMP00000034734

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage