LATS2 Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF804435

Carrier-free (BSA/glycerol-free) LATS2 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LATS2 (RC219394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LATS2. Positive lysates LY402348 (100ug) and LC402348 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-237 of human LATS2 (NP_055387) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 120 kDa
• Gene Name: Homo sapiens large tumor suppressor kinase 2 (LATS2), mRNA.
• Database Link:
  NP_055387
  Entrez Gene 50523 MouseEntrez Gene 26524 Human
  UniProt ID Q9NRM7
• Background: This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]
• Synonyms: KPM

Reference Data

• Protein Families: Druggable Genome, Protein Kinase