Cardiac Troponin I (TNNC1) Mouse Monoclonal Antibody [Clone ID: OTI5H3]

CAT#: CF804479

Carrier-free (BSA/glycerol-free) TNNC1 mouse monoclonal antibody, clone OTI5H3 (formerly 5H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNC1 (RC207014, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNC1. Positive lysates LY401131 (100ug) and LC401131 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804479)

Specifications

Product Data

• Clone Name: OTI5H3
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TNNC1 (NP_003271) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.2 kDa
• Gene Name: Homo sapiens troponin C1, slow skeletal and cardiac type (TNNC1), mRNA.
• Database Link:
  NP_003271
  Entrez Gene 21924 MouseEntrez Gene 290561 RatEntrez Gene 7134 Human
  UniProt ID P63316
• Background: Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]
• Synonyms: CMD1Z; CMH13; TN-C; TNC; TNNC

Reference Data

• Protein Pathways: Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)