RRM1 Mouse Monoclonal Antibody [Clone ID: OTI16A8]

CAT#: CF804537

Carrier-free (glycerol/BSA-free) RRM1 mouse monoclonal antibody, clone OTI16A8 (formerly 16A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1 (RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1. Positive lysates LY422336 (100ug) and LC422336 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RRM1 Mouse monoclonal antibody. (TA804537; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RRM1 Mouse monoclonal antibody. (TA804537; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Specifications

Product Data

• Clone Name: OTI16A8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 541-792 of human RRM1 (NP_001024) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89.9 kDa
• Gene Name: Homo sapiens ribonucleotide reductase catalytic subunit M1 (RRM1), transcript variant 1, mRNA.
• Database Link:
  NP_001024
  Entrez Gene 20133 MouseEntrez Gene 685579 RatEntrez Gene 6240 Human
  UniProt ID P23921
• Background: This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq, Jul 2008]
• Synonyms: R1; RIR1; RR1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, Purine metabolism, Pyrimidine metabolism