PAFAH1B3 Mouse Monoclonal Antibody [Clone ID: OTI4B2]

CAT#: CF804553

Carrier-free (BSA/glycerol-free) Purified PAFAH1B3 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAFAH1B3 (RC227227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAFAH1B3. Positive lysates LY429044 (100ug) and LC429044 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4B2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PAFAH1B3 (NP_001139411) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.6 kDa
• Gene Name: Homo sapiens platelet activating factor acetylhydrolase 1b catalytic subunit 3 (PAFAH1B3), transcript variant 1, mRNA.
• Database Link:
  NP_001139411
  Entrez Gene 5050 Human
  UniProt ID Q15102
• Background: This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with mental retardation, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
• Synonyms: PAFAHG

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ether lipid metabolism, Metabolic pathways