Periostin (POSTN) Mouse Monoclonal Antibody [Clone ID: OTI2B2]

CAT#: CF804575

Carrier-free (BSA/glycerol-free) POSTN mouse monoclonal antibody, clone OTI2B2 (formerly 2B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POSTN (RC227934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POSTN. Positive lysates LY427734 (100ug) and LC427734 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804575)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804575)

• 

  Figure from citation: Immunohistochemical staining for POSTN protein of negative control, positive control, enchondroma, and low grade chondrosarcoma (original magnification ×400) by using anti-POSTN antibody. Dilution: 1:1000 View Citation

Specifications

Product Data

• Clone Name: OTI2B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 538-781 of human POSTN (NP_001129407) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87.1 kDa
• Gene Name: Homo sapiens periostin (POSTN), transcript variant 3, mRNA.
• Database Link:
  NP_001129407
  Entrez Gene 50706 MouseEntrez Gene 10631 Human
  UniProt ID Q15063
• Synonyms: OSF-2; OSF2; PDLPOSTN; PN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein