RSPO3 Mouse Monoclonal Antibody [Clone ID: OTI3D5]

CAT#: CF804633

Carrier-free (glycerol/BSA-free) RSPO3 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RSPO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804633)

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Specifications

Product Data

• Clone Name: OTI3D5
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-272 of human RSPO3 (NP_116173) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: Homo sapiens R-spondin 3 (RSPO3), mRNA.
• Database Link:
  NP_116173
  Entrez Gene 72780 MouseEntrez Gene 498997 RatEntrez Gene 84870 Human
  UniProt ID Q9BXY4
• Synonyms: CRISTIN1; PWTSR; THSD2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein