CLOCK Mouse Monoclonal Antibody [Clone ID: OTI2C3]

CAT#: CF804773

Carrier-free (BSA/glycerol-free) CLOCK mouse monoclonal antibody, clone OTI2C3 (formerly 2C3)

Product Images

Human recombinant protein fragment corresponding to amino acids 1-285 of human CLOCK (NP_004889) produced in E.coli.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CLOCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804773)

Specifications

Product Data

• Clone Name: OTI2C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-285 of human CLOCK (NP_004889) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 95.1 kDa
• Gene Name: Homo sapiens clock circadian regulator (CLOCK), transcript variant 2, mRNA.
• Database Link:
  NP_004889
  Entrez Gene 12753 MouseEntrez Gene 60447 RatEntrez Gene 9575 Human
  UniProt ID O15516
• Synonyms: bHLHe8; KAT13D

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal