NIPP1 (PPP1R8) Mouse Monoclonal Antibody [Clone ID: OTI5H9]

CAT#: CF804813

Carrier-free (BSA/glycerol-free) PPP1R8 mouse monoclonal antibody, clone OTI5H9 (formerly 5H9)

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804813)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804813)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804813)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804813)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804813)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804813)

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Specifications

Product Data

• Clone Name: OTI5H9
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-225 of human PPP1R8 (NP_612568) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.5 kDa
• Gene Name: Homo sapiens protein phosphatase 1 regulatory subunit 8 (PPP1R8), transcript variant 2, mRNA.
• Database Link:
  NP_612568
  Entrez Gene 100336 MouseEntrez Gene 313030 RatEntrez Gene 5511 Human
  UniProt ID Q12972
• Background: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047

Reference Data

• Protein Families: Druggable Genome, Transcription Factors