ECE1 Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CAT#: CF804823

Carrier-free (glycerol/BSA-free) ECE1 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECE1 (RC223135, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECE1. Positive lysates LY419958 (100ug) and LC419958 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 486-770 of human ECE1 (NP_001388) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87 kDa
• Gene Name: Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.
• Database Link:
  NP_001388
  Entrez Gene 94204 RatEntrez Gene 230857 MouseEntrez Gene 1889 Human
  UniProt ID P42892
• Background: The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep
• Synonyms: ECE

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane