Integrin Linked Kinase (ILK) Mouse Monoclonal Antibody [Clone ID: OTI2H3]

CAT#: CF804883

Carrier-free (BSA/glycerol-free) ILK mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILK (RC201544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILK. Positive lysates LY401438 (100ug) and LC401438 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2H3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-244 of human ILK (NP_004508) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.2 kDa
• Gene Name: Homo sapiens integrin linked kinase (ILK), transcript variant 1, mRNA.
• Database Link:
  NP_004508
  Entrez Gene 16202 MouseEntrez Gene 170922 RatEntrez Gene 3611 Human
  UniProt ID Q13418
• Synonyms: HEL-S-28; ILK-1; ILK-2; P59; p59ILK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endometrial cancer, Focal adhesion, PPAR signaling pathway