PDGF Receptor alpha (PDGFRA) Mouse Monoclonal Antibody [Clone ID: OTI2B12]

CAT#: CF804957

Carrier-free (BSA/glycerol-free) PDGFRA mouse monoclonal antibody, clone OTI2B12 (formerly 2B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA. Positive lysates LY416803 (100ug) and LC416803 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-324 of human PDGFRA (NP_006197) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 120.3 kDa
• Gene Name: Homo sapiens platelet derived growth factor receptor alpha (PDGFRA), transcript variant 1, mRNA.
• Database Link:
  NP_006197
  Entrez Gene 18595 MouseEntrez Gene 25267 RatEntrez Gene 5156 Human
  UniProt ID P16234
• Synonyms: CD140A; PDGFR-2; PDGFR2; RHEPDGFRA

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• Protein Pathways: Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton