SAE1 Mouse Monoclonal Antibody [Clone ID: OTI3C11]

CAT#: CF805140

Carrier-free (BSA/glycerol-free) SAE1 mouse monoclonal antibody, clone OTI3C11 (formerly 3C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (RC201820, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1. Positive lysates LY401688 (100ug) and LC401688 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3C11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAE1 (NP_005491) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mRNA.
• Database Link:
  NP_005491
  Entrez Gene 56459 MouseEntrez Gene 308384 RatEntrez Gene 10055 Human
  UniProt ID Q9UBE0
• Synonyms: AOS1; HSPC140; SUA1; UBLE1A

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis