Constitutive androstane receptor (NR1I3) Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF805146

Carrier-free (BSA/glycerol-free) NR1I3 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3 (RC213905, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1I3. Positive lysates LY425868 (100ug) and LC425868 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-NR1I3 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NR1I3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805146)

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-236 of human NR1I3 (NP_001070950) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.3 kDa
• Gene Name: Homo sapiens nuclear receptor subfamily 1 group I member 3 (NR1I3), transcript variant 1, mRNA.
• Database Link:
  NP_001070950
  Entrez Gene 9970 Human
  UniProt ID Q14994
• Background: This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CAR; CAR1; MB67

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors