Nkx3.1 (NKX3-1) Mouse Monoclonal Antibody [Clone ID: OTI2A5]

CAT#: CF805152

Carrier-free (BSA/glycerol-free) NKX3-1 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX3-1 (RC210374, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKX3-1. Positive lysates LY401858 (100ug) and LC401858 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NKX3-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805152)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NKX3-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805152)

Specifications

Product Data

• Clone Name: OTI2A5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NKX3-1 (NP_006158) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.2 kDa
• Gene Name: Homo sapiens NK3 homeobox 1 (NKX3-1), transcript variant 1, mRNA.
• Database Link:
  NP_006158
  Entrez Gene 4824 Human
  UniProt ID Q99801
• Background: This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]
• Synonyms: BAPX2; NKX3; NKX3.1; NKX3A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Prostate cancer