TMEM16A (ANO1) Mouse Monoclonal Antibody [Clone ID: OTI1C8]

CAT#: CF805184

Carrier-free (BSA/glycerol-free) ANO1 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)

Product Images

Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

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  Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

• Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805184)

• Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA805184) (1:1000)

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Specifications

Product Data

• Clone Name: OTI1C8
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-333 of human ANO1 (NP_060513) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA.
• Database Link:
  NP_060513
  Entrez Gene 101772 MouseEntrez Gene 309135 RatEntrez Gene 55107 Human
  UniProt ID Q5XXA6
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A

Reference Data

• Protein Families: Transmembrane