ADAMTS4 Mouse Monoclonal Antibody [Clone ID: OTI6F8]

CAT#: CF805208

Carrier-free (BSA/glycerol-free) ADAMTS4 mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADAMTS4 (RC209226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADAMTS4. Positive lysates LY417514 (100ug) and LC417514 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ADAMTS4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA805208) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ADAMTS4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA805208) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ADAMTS4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA805208) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI6F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 296-577 of human ADAMTS4 (NP_005090) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 4 (ADAMTS4), transcript variant 1, mRNA.
• Database Link:
  NP_005090
  Entrez Gene 66015 RatEntrez Gene 240913 MouseEntrez Gene 9507 Human
  UniProt ID O75173
• Synonyms: ADAMTS-2; ADAMTS-4; ADMP-1

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane