TFPI2 Mouse Monoclonal Antibody [Clone ID: OTI1D4]

CAT#: CF805209

Carrier-free (BSA/glycerol-free) TFPI2 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI2 (RC202760, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI2. Positive lysates LY416564 (100ug) and LC416564 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TFPI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805209)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TFPI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805209)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TFPI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805209)

Specifications

Product Data

• Clone Name: OTI1D4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 23-235 of human TFPI2 (NP_006519) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.8 kDa
• Gene Name: Homo sapiens tissue factor pathway inhibitor 2 (TFPI2), transcript variant 1, mRNA.
• Database Link:
  NP_006519
  Entrez Gene 7980 Human
  UniProt ID P48307
• Background: This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
• Synonyms: PP5; REF1; TFPI-2

Reference Data

• Protein Families: Secreted Protein