TRIB1 Mouse Monoclonal Antibody [Clone ID: OTI8C8]

CAT#: CF805274

Carrier-free (BSA/glycerol-free) TRIB1 mouse monoclonal antibody, clone OTI8C8 (formerly 8C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB1 (RC209219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB1. Positive lysates LY410846 (100ug) and LC410846 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805274)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TRIB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805274)

Specifications

Product Data

• Clone Name: OTI8C8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TRIB1 (NP_079471) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens tribbles pseudokinase 1 (TRIB1), transcript variant 1, mRNA.
• Database Link:
  NP_079471
  Entrez Gene 78969 RatEntrez Gene 211770 MouseEntrez Gene 10221 Human
  UniProt ID Q96RU8
• Synonyms: C8FW; GIG-2; GIG2; SKIP1; TRB-1; TRB1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase