Apolipoprotein E (APOE) Mouse Monoclonal Antibody [Clone ID: OTI2A4]

CAT#: CF805331

Carrier-free (BSA/glycerol-free) APOE mouse monoclonal antibody, clone OTI2A4 (formerly 2A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOE (RC200395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOE (1:500). Positive lysates LY424959 (100ug) and LC424959 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805331) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human placenta tissue using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805331) (1:150)

Specifications

Product Data

• Clone Name: OTI2A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human APOE (NP_000032) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.2 kDa
• Gene Name: Homo sapiens apolipoprotein E (APOE), transcript variant 2, mRNA.
• Database Link:
  NP_000032
  Entrez Gene 348 Human
  UniProt ID P02649
• Background: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jul 2008]
• Synonyms: AD2; APO-E; LDLCQ5; LPG

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
• Protein Pathways: Alzheimer's disease