CDKL2 Mouse Monoclonal Antibody [Clone ID: OTI12C2]

CAT#: CF805580

Carrier-free (BSA/glycerol-free) CDKL2 mouse monoclonal antibody, clone OTI12C2 (formerly 12C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKL2 (RC210780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKL2. Positive lysates LY418330 (100ug) and LC418330 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDKL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805580)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CDKL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805580)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CDKL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805580)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CDKL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805580)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDKL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805580)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDKL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805580)

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Specifications

Product Data

• Clone Name: OTI12C2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 291-493 of human CDKL2 (NP_003939) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.8 kDa
• Gene Name: Homo sapiens cyclin dependent kinase like 2 (CDKL2), transcript variant 1, mRNA.
• Database Link:
  NP_003939
  Entrez Gene 53886 MouseEntrez Gene 305242 RatEntrez Gene 8999 Human
  UniProt ID Q92772
• Background: This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the cytoplasm, with lower levels in the nucleus. [provided by RefSeq, Jul 2008]
• Synonyms: KKIAMRE; P56

Reference Data

• Protein Families: Druggable Genome, Protein Kinase